Determination of Antioxidant Activity of Crambe Cordifolia

In the present investigation, the antioxidant activity and free radical scavenging activity of Crambe cordifolia plant were spectrophotometrically studied. Free radical scavenging activity was determined by DPPH method and the obtained IC value was 1106.65 μg/mL. For comparison, the IC value of standard ascorbic acid 50 50 was 21.69 μg/mL. Antioxidant activity was determined by phosphomolybdate method and the results showed that the plant had 205.73 μg per gram -tocopherol equivalent antioxidant content. Spectrophotometric determination of phenolic and flavonoid content was also carried out in terms of pyrocatechol equivalent and quercetin equivalent, respectively. Plant contained 10.60 μg pyrocatechol equivalent phenolic content and 1.43 μg quercetin equivalent flavonoid content per gram of plant material. The results indicated that Crambe cordifolia was a potent source of natural antioxidants.